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KMID : 0613820070170040482
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Journal of Life Science
2007 Volume.17 No. 4 p.482 ~ p.492
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Regulation of Gb3 Expression on Dendritic Cells
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Lim Suk-Hwan
Kim Ki-Young
Kim Hyung-Chun
Kim Young-Hee
Son Yong-Hae
Oh Yang-Hyo
Park Young-Min
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Abstract
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Infection with Shiga-like toxin (SLT)-producing Escherichia coli causes a spectrum of illnesses with high morbidity and mortality. Host mediators play an important role in the pathogenesis of SLT-I toxicity. We here investigated the effect of SLT-I on tumor necrosis factor-α (TNF-α) production, effect of TNF-α on glycolipid globotriaosyleramide (Gb3) expression, and relationship between Gb3 level and differential susceptibility of cells to SLT-I. In this study, we observed that detectable levels of TNF-α are produced 6 hrs after induction and continued to increase during 48 hrs by SLT-I. It was also found that Vero cells and dendritic cells expressed high levels of Gb3, 83% and 68%, respectively, and that macrophages had a low level of Gb3 (29%) and showed refractory to cytotoxicity against SLT-I. Vero cells and dendritic cells expressing high levels of Gb3 were highly susceptible to SLT-I. Furthermore, macrophages showed a resistance to SLT-I cytotoxicity, despite the fact that Gb3 expression was enhanced. These results suggest that the expression of Gb3 is necessary, but not sufficient to confer sensitivity of macrophages to SLT-I and further underpin the important role of SLT-I and its receptor, Gb3, in the pathogenesis of E. coli O157 infection.
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KEYWORD
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E. coli O157, Shiga-like toxin, tumor necrosis factor-α, glycolipid globotriaosy leramide
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